Identification of Verticillium species and V. longisporum lineages

We suggest the following four steps (1) to identify Verticillium species and distinguish among the lineages of V. longisporum:

- Morphology-based identification using a dichotomous key (3);
- Confirmation of the initial identification with appropriate diagnostic PCR assays (2, 4);
- Blast search against a reference set of phylogenetically informative sequences at VertShield, with one or more loci;
- For an isolate that appears to be a new species, conduct thorough phylogenetic analyses using sequences of multiple loci.

The results from identification efforts and the reasoning for selection of a particular name should be reported in publications. Also, to guarantee continuity of research, please deposit sequences to GenBank and cultures and DNA extracts to public collections.

References

1. Inderbitzin, P., and Subbarao, K. V. 2014. Verticillium systematics and evolution: how confusion impedes Verticillium wilt management and how to resolve it. Phytopathology 104:564-574.
2. Inderbitzin, P., Davis, R. M., Bostock, R. M., and Subbarao, K. V. 2013. Identification and differentiation of Verticillium species and V. longisporum lineages by simplex and multiplex PCR assays. PLoS ONE 8:e65990.
3. Inderbitzin, P., Bostock, R. M., Davis, R. M., Usami, T., Platt, H. W., and Subbarao, K. V. 2011. Phylogenetics and taxonomy of the fungal vascular wilt pathogen Verticillium, with the descriptions of five new species. PLoS ONE 6:e28341.
4. Tran, V., Braus-Stromeyer, S. A., Timpner, C., and Braus, G. H. 2013. Molecular diagnosis to discriminate pathogen and a pathogen species of the hybrid Verticillium longisporum on the oilseed crop Brassica napus. Appl. Microbiol. Biotechnol. 97:4467-4483.