Multiplex PCR assay to differentiate V. albo-atrum, V. alfalfae and V. nonalfalfae


Diagnostic band sizes, primers and target loci
Species Size Primers Loci
V. albo-atrum 725bp AaF / AaTr ACT
V. alfalfae 1060bp AlfF / AlfD1r GPD
V. nonalfalfae 1310bp NoF / NoNuR TS
Primer sequences
AaF5'-GGCCTCGATAGCATCGCC
AlfD1r5'-TGCCGGCATCGACCTTGG
AlfF5'-TCATGCCCCCTTTGTTCATCGAT
AaTr5'-CTGGATGGAGACGTAGAAGGC
NoF5'-CCTCGAAAAATCCACCAGCTCTA
NoNuR5'-GTGGTTGAGATCCTCACGCTTC

Reference

Inderbitzin, P., Davis, R. M., Bostock, R. M., and Subbarao, K. V. 2013. Identification and differentiation of Verticillium species and V. longisporum lineages by simplex and multiplex PCR assays.





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