Multiplex PCR assay to differentiate V. dahliae, V. isaacii, V. klebahnii and V. tricorpus


Diagnostic band sizes, primers and target loci
Species Size Primers Loci
V. dahliae 490bp Df / Dr ITS
V. isaacii 490bp If / IKr EF
V. klebahnii 230bp Kf / IKr EF
V. tricorpus 415bp Tf / aaTr ACT
Primer sequences
AaTr5'-CTGGATGGAGACGTAGAAGGC
Df5'-CCGGTCCATCAGTCTCTCTG
Dr5'-CTGTTGCCGCTTCACTCG
If5'-CGATGTCGCGATGACCTCG
IKr5'-CGGCAGCCTCCTAAACATGG
Kf5'-ACATCCTGAGGCTGCTTGAGA
Tf5'-CGTGCTGTCTTCCGTAAGTTTG

Reference

Inderbitzin, P., Davis, R. M., Bostock, R. M., and Subbarao, K. V. 2013. Identification and differentiation of Verticillium species and V. longisporum lineages by simplex and multiplex PCR assays. PLoS ONE 8:e65990. http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0065990





Back