Multiplex PCR assay to differentiate V. dahliae, and the three V. longisporum lineages




Primer sequences
A1f5'-AAGTGGAGCCCCGTATCTTGAAT
A1r5'-CAACTGGCAACAGGGCTTGAAT
AlfD1r5'-TGCCGGCATCGACCTTGG
Df5'-CCGGTCCATCAGTCTCTCTG
Dr5'-CTGTTGCCGCTTCACTCG
D1f5'-CCCCGGCCTTGGTCTGAT

Reference

Inderbitzin, P., Davis, R. M., Bostock, R. M., and Subbarao, K. V. 2013. Identification and differentiation of Verticillium species and V. longisporum lineages by simplex and multiplex PCR assays. PLoS ONE 8:e65990. http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0065990





Back