Multiplex PCR assay to differentiate V. isaacii, and the three V. klebahnii and V. tricorpus




Primer sequences
AaTr5'-CTGGATGGAGACGTAGAAGGC
If5'-CGATGTCGCGATGACCTCG
IKr5'-CGGCAGCCTCCTAAACATGG
Kf5'-ACATCCTGAGGCTGCTTGAGA
Tf5'-CGTGCTGTCTTCCGTAAGTTTG

Reference

Inderbitzin, P., Davis, R. M., Bostock, R. M., and Subbarao, K. V. 2013. Identification and differentiation of Verticillium species and V. longisporum lineages by simplex and multiplex PCR assays. PLoS ONE 8:e65990. http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0065990


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